pc10 (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
pc10
Pc10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 6710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcna+pc10/PCNA+Antibody/pmc12813500-95-19-20
Average 96 stars, based on 6710 article reviews
Pc10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 6710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcna+pc10/PCNA+Antibody/pmc12813500-95-19-20
Average 96 stars, based on 6710 article reviews
pc10 - by Bioz Stars,
2026-09
96/100 stars
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Immunostaining:Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 minutes, permeabilised in cold methanol (Sigma Aldrich) for 2 minutes, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e., Tris Buffered Saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4°C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures. Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 min, permeabilised in cold methanol (Sigma Aldrich) for 2 min, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e. Tris buffered saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4 °C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 min, permeabilised in cold methanol (Sigma Aldrich) for 2 min, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e. Tris buffered saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4 °C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Incubation:Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 minutes, permeabilised in cold methanol (Sigma Aldrich) for 2 minutes, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e., Tris Buffered Saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4°C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures. Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 min, permeabilised in cold methanol (Sigma Aldrich) for 2 min, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e. Tris buffered saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4 °C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 min, permeabilised in cold methanol (Sigma Aldrich) for 2 min, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e. Tris buffered saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4 °C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Blocking Assay:Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 minutes, permeabilised in cold methanol (Sigma Aldrich) for 2 minutes, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e., Tris Buffered Saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4°C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures. Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 min, permeabilised in cold methanol (Sigma Aldrich) for 2 min, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e. Tris buffered saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4 °C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures Article Snippet: After treatments, cells were washed in DPBS (Gibco, #2037539), fixed in 4% paraformaldehyde solution (Histolab, 02176) for 7 min, permeabilised in cold methanol (Sigma Aldrich) for 2 min, washed again in DPBS and blocked with 2% BSA (Sigma-Aldrich A9418) in TBS/T (i.e. Tris buffered saline solution supplemented with 0.1% Tween-20). .. If immunostaining applied, fixed samples were incubated overnight at 4 °C with a primary antibody: phospho-histone H2A.X [Ser139] (1:800, Cell Signalling Technology, #2577); phospho-RPA32 [S33] (1:2000, Bethyl Laboratories, #A300-246A); or Staining:Article Title: Variety in the USP deubiquitinase catalytic mechanism Article Snippet: Proteins were transferred to a nitrocellulose membrane (10600002; Amersham Protran 0.45 NC nitrocellulose). .. Membranes were stained with the following antibodies: Ubiquityl-PCNA (Lys164) (D5C7P) (13439; Cell Signaling Technology), Article Title: ADAR1 exacerbates ischemic brain injury via astrocyte-mediated neuron apoptosis. Article Snippet: 2,3,5-triphenyl-tetrazolium chloride solution (TTC) and crystal violet (C0775) were purchased from Millipore Sigma (St. Louis, MO). .. The following antibodies were used in Western blotting and immunofluorescent staining: ADAR1 (D-8, for western blotting), other:Article Title: Profiling of microglia nodules in multiple sclerosis reveals propensity for lesion formation Article Snippet: PCNA , Western Blot:Article Title: ADAR1 exacerbates ischemic brain injury via astrocyte-mediated neuron apoptosis. Article Snippet: 2,3,5-triphenyl-tetrazolium chloride solution (TTC) and crystal violet (C0775) were purchased from Millipore Sigma (St. Louis, MO). .. The following antibodies were used in Western blotting and immunofluorescent staining: ADAR1 (D-8, for western blotting), Inhibition:Article Title: Apocynin may alleviate side effects of autophagy-blocked radiotherapy through antioxidant effects. Article Snippet: One of the most widely used techniques for cancer treatment is radiotherapy.. Autophagic pathways allow some cancer cells that are resistant to radiation therapy to survive.. Inhibiting autophagy has been shown to improve radiotherapy efficacy in several cancer types. |